Direct detection of Borrelia burgdorferi spirochetes in patients with early,diss

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Direct detection of Borrelia burgdorferi spirochetes

in patients with early,disseminated Lyme borreliosis.

 

Cent Eur J Public Health. 2009 Dec;17(4):179-82.

 

Direct detection of Borrelia burgdorferi spirochetes in patients

with early disseminated Lyme borreliosis.

 

Schwarzova K, Kost'anova Z, Holeckova K, Spitalska E, Boldis V.

 

Institute of Virology, Slovak Academy of Sciences, Bratislava.

virukats

 

The detection of spirochetes in 15 patients with clinically

documented early disseminated LB has been analysed when using

cultivation method of the plasma or the cerebrospinal fluid,

electron microscopy, commercial Western blot and detecting the

DNA of the pathogen in vitro cultures by PCR-RFLP. Spirochetes

were isolated in eight blood and one cerebrospinal fluid culture

samples.

 

*In seven cases (47%), previous serodiagnostic laboratory tests

were negative*

 

Borrelial DNA was detected by PCR in 67% patients (9 blood

samples and 1 CSF sample). Using Msel restriction fragments of

PCR products of the amplified rrf-rrl region, we identified

Borrelia garinii (80%), one B. afzelii isolate and one B.

burgdorferi s.s.

 

Publication Types:

Research Support, Non-U.S. Gov't

 

http://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pu

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PMID: 20377044 [PubMed - in process]