Guest guest Posted April 18, 2004 Report Share Posted April 18, 2004 J.P. Shah,1* P.Midkiff,1 P.C. Brandt,2 and B.F. Sisken1* Bioelectromagnetics 22:267-271 (2001) Growth and Differentiation of PC6 Cells: the Effects of Pulsed Electromagnetic Fields (PEMF) 1Center for Biomedical Engineering and Department ofAnatomy and Neurobiology, 2Department of Biochemistry, University of Kentucky, Lexington, Kentucky Previous studies in our laboratory showed that neurite outgrowth in vitro and nerve regeneration in vivo were stimulated by 2 Hz, 0.3mT (3 G) pulsed electromagnetic fields (PEMF). To learn more about the effects of PEMF on nerve cells, we exposed PC6 cells, a standard neuronal-like cell model, to the same pulsed electromagnetic fields for 2 h/day for 2 days and asked whether two different cell processes, proliferation and differentiation, were affected. The cells were also treated with a differentiating agent, nerve growth factor (NGF), to further de®ne any interactive effects. We found that proliferation was unaffected by either PEMF or NGF alone or in combination. Differentiation, expressed as neurite outgrowth, was strongly upregulated with NGF, but this NGF response was significantly depressed in cells treated with PEMF. F.M. Longo,1* T. Yang,1 S. Hamilton,2,3 J.F. Hyde,3 J.Walker,2,4 L. Jennes,3 R. Stach,5 and B.F. Sisken2,3 Electromagnetic Fields Influence NGFActivity and Levels Following Sciatic Nerve Transection. J. Neurosci. Res. 55:230–237, 1999. Published 1999 Wiley-Liss, Inc. 1Department of Neurology, UCSF/VAMC, San Francisco, California 2Center for Biomedical Engineering, University of Kentucky, Lexington 3Department of Anatomy and Neurobiology, University of Kentucky, Lexington 4Division of Orthopedic Surgery, University of Kentucky, Lexington 5Department of Chemistry, University of Michigan, Flint Pulsed electromagnetic fields (PEMF) have been shown to increase the rate of nerve regeneration. Transient post-transection loss of target- derived nerve growth factor (NGF) is one mechanism proposed to signal induction of early nerve regenerative events. We tested the hypothesis that PEMF alter levels of NGF activity and protein in injured nerve and/or dorsal root ganglia (DRG) during the first stages of regeneration (6–72 hr). Rats with a transection injury to the midthigh portion of the sciatic nerve on one side were exposed to PEMF or sham control PEMF for 4 hr/day for different time periods. NGF-like activity was determined in DRG, in 5-mm nerve segments proximal and distal to the transection site and in a corresponding 5-mm segment of the contralateral nonoperated nerve. NGF-like activity of coded tissue samples was measured in a blinded fashion using the chick DRG sensory neuron bioassay. Overall, PEMF caused a significant decrease in NGF-like activity in nerve tissue (P<.02, repeated measures analysis of variance, ANOVA) with decreases evident in proximal, distal, and contralateral nonoperated nerve. Unexpectedly, transection was also found to cause a significant (P<.001) 2-fold increase in DRG NGFlike activity between 6 and 24 hr postinjury in contralateral but not ipsilateral DRG. PEMF also reduced NGF-like activity in DRG, although this decrease did not reach statistical significance. Assessment of the same nerve and DRG samples using ELISA and NGF-specific antibodies confirmed an overall significant (P F 0.001) decrease in NGF levels in PEMF-treated nerve tissue, while no decrease was detected in DRG or in nerve samples harvested from PEMF-treated uninjured rats. These findings demonstrate that PEMF can affect growth factor activity and levels, and raise the possibility that PEMF might promote nerve regeneration by amplifying the early postinjury decline in NGF activity. Best regards, Email: < WORK : Teagasc Research Management, Sandymount Ave., Dublin 4, Ireland Mobile: 353-; [in the Republic: 0] HOME : 1 Esker Lawns, Lucan, Dublin, Ireland Tel : 353-; [in the Republic: 0] WWW : http://homepage.eircom.net/~progers/searchap.htm Quote Link to comment Share on other sites More sharing options...
Guest guest Posted April 19, 2004 Report Share Posted April 19, 2004 Hi Phil! Interesting, I think . . . Just curious, does your keyboard have a " tab " key? An " enter " key? If you do have these available to you, you *could* use them to clarify your postings. Just a thought. At 02:25 PM 4/18/2004, you wrote: >J.P. Shah,1* P.Midkiff,1 P.C. Brandt,2 and B.F. Sisken1* >Bioelectromagnetics 22:267-271 (2001) Growth and Differentiation >of PC6 Cells: the Effects of Pulsed Electromagnetic Fields (PEMF) >1Center for Biomedical Engineering and Department ofAnatomy >and Neurobiology, 2Department of Biochemistry, University of Regards, Pete Quote Link to comment Share on other sites More sharing options...
Guest guest Posted April 19, 2004 Report Share Posted April 19, 2004 Are we inferring (or should we?) a connection between acupuncture and PEMF and if so is this only for acupuncture with e-stim? I have a number of prospective patients with post surgical (carpal tunnel in the main) numbness and tingling due to nerve damage - this is 12 months post surgery and no improvement. Kayte Halstead Acupuncture 4 Health Chinese Medicine , " " <@e...> wrote: > J.P. Shah,1* P.Midkiff,1 P.C. Brandt,2 and B.F. Sisken1* > Bioelectromagnetics 22:267-271 (2001) Growth and Differentiation > of PC6 Cells: the Effects of Pulsed Electromagnetic Fields (PEMF) > 1Center for Biomedical Engineering and Department ofAnatomy > and Neurobiology, 2Department of Biochemistry, University of > Kentucky, Lexington, Kentucky Previous studies in our laboratory > showed that neurite outgrowth in vitro and nerve regeneration in vivo > were stimulated by 2 Hz, 0.3mT (3 G) pulsed electromagnetic > fields (PEMF). To learn more about the effects of PEMF on nerve > cells, we exposed PC6 cells, a standard neuronal-like cell model, > to the same pulsed electromagnetic fields for 2 h/day for 2 days > and asked whether two different cell processes, proliferation and > differentiation, were affected. The cells were also treated with a > differentiating agent, nerve growth factor (NGF), to further de®ne > any interactive effects. We found that proliferation was unaffected > by either PEMF or NGF alone or in combination. Differentiation, > expressed as neurite outgrowth, was strongly upregulated with > NGF, but this NGF response was significantly depressed in cells > treated with PEMF. > > F.M. Longo,1* T. Yang,1 S. Hamilton,2,3 J.F. Hyde,3 J.Walker,2,4 > L. Jennes,3 R. Stach,5 and B.F. Sisken2,3 Electromagnetic Fields > Influence NGFActivity and Levels Following Sciatic Nerve > Transection. J. Neurosci. Res. 55:230–237, 1999. Published 1999 > Wiley-Liss, Inc. 1Department of Neurology, UCSF/VAMC, San > Francisco, California 2Center for Biomedical Engineering, > University of Kentucky, Lexington 3Department of Anatomy and > Neurobiology, University of Kentucky, Lexington 4Division of > Orthopedic Surgery, University of Kentucky, Lexington > 5Department of Chemistry, University of Michigan, Flint Pulsed > electromagnetic fields (PEMF) have been shown to increase the > rate of nerve regeneration. Transient post-transection loss of target- > derived nerve growth factor (NGF) is one mechanism proposed to > signal induction of early nerve regenerative events. We tested the > hypothesis that PEMF alter levels of NGF activity and protein in > injured nerve and/or dorsal root ganglia (DRG) during the first > stages of regeneration (6–72 hr). Rats with a transection injury to > the midthigh portion of the sciatic nerve on one side were exposed > to PEMF or sham control PEMF for 4 hr/day for different time > periods. NGF-like activity was determined in DRG, in 5-mm nerve > segments proximal and distal to the transection site and in a > corresponding 5-mm segment of the contralateral nonoperated > nerve. NGF-like activity of coded tissue samples was measured in > a blinded fashion using the chick DRG sensory neuron bioassay. > Overall, PEMF caused a significant decrease in NGF-like activity in > nerve tissue (P<.02, repeated measures analysis of variance, > ANOVA) with decreases evident in proximal, distal, and > contralateral nonoperated nerve. Unexpectedly, transection was > also found to cause a significant (P<.001) 2-fold increase in DRG > NGFlike activity between 6 and 24 hr postinjury in contralateral but > not ipsilateral DRG. PEMF also reduced NGF-like activity in DRG, > although this decrease did not reach statistical significance. > Assessment of the same nerve and DRG samples using ELISA > and NGF-specific antibodies confirmed an overall significant (P F > 0.001) decrease in NGF levels in PEMF-treated nerve tissue, while > no decrease was detected in DRG or in nerve samples harvested > from PEMF-treated uninjured rats. These findings demonstrate that > PEMF can affect growth factor activity and levels, and raise the > possibility that PEMF might promote nerve regeneration by > amplifying the early postinjury decline in NGF activity. > Best regards, > > Email: <@e...> > > WORK : Teagasc Research Management, Sandymount Ave., Dublin 4, Ireland > Mobile: 353-; [in the Republic: 0] > > HOME : 1 Esker Lawns, Lucan, Dublin, Ireland > Tel : 353-; [in the Republic: 0] > WWW : http://homepage.eircom.net/~progers/searchap.htm Quote Link to comment Share on other sites More sharing options...
Guest guest Posted April 19, 2004 Report Share Posted April 19, 2004 Hi Pete > Hi Phil! Just curious, does your keyboard have a " tab " key? An > " enter " key? Yes, I have standard keyboard. > If you do have these available to you, you *could* use them to > > clarify your postings. Just a thought. At 02:25 PM 4/18/2004, you > wrote: J.P. Shah,1* P.Midkiff,1 P.C. Brandt,2 and B.F. Sisken1* > Bioelectromagnetics 22:267-271 (2001) Growth and Differentiation > of PC6 Cells: the Effects of Pulsed Electromagnetic Fields > (PEMF) 1Center for Biomedical Engineering and Department > ofAnatomy and Neurobiology, 2Department of Biochemistry, > University of ... Regards, Pete How were my postings not clear? Best regards, Email: < WORK : Teagasc Research Management, Sandymount Ave., Dublin 4, Ireland Mobile: 353-; [in the Republic: 0] HOME : 1 Esker Lawns, Lucan, Dublin, Ireland Tel : 353-; [in the Republic: 0] WWW : http://homepage.eircom.net/~progers/searchap.htm Quote Link to comment Share on other sites More sharing options...
Guest guest Posted April 19, 2004 Report Share Posted April 19, 2004 Hi Kayte, > Are we inferring (or should we?) a connection between acupuncture > and PEMF and if so is this only for acupuncture with e-stim? IMO, acupoints can be activated by many different stimuli: needling, guasha, electro-AP, magnets, Dermajet, TENS, e-stim, ultrasound, colour, laser, massage, heat, cold, etc, etc. IMO, the POINTS are more important than the STIMULUS applied. PEMF is only one stimulus, but simple needling could be as effective, IMO. > I have a number of prospective patients with post surgical (carpal > tunnel in the main) numbness and tingling due to nerve damage - > this is 12 months post surgery and no improvement. Kayte IMO, simple AP at points along the affected channel + related points (as in Richard Tan's method, could be useful here. Treat as for peripheral (sensory) nerve paralysis. Best regards, Email: < WORK : Teagasc Research Management, Sandymount Ave., Dublin 4, Ireland Mobile: 353-; [in the Republic: 0] HOME : 1 Esker Lawns, Lucan, Dublin, Ireland Tel : 353-; [in the Republic: 0] WWW : http://homepage.eircom.net/~progers/searchap.htm Quote Link to comment Share on other sites More sharing options...
Guest guest Posted April 19, 2004 Report Share Posted April 19, 2004 Phil - IMO treating the nerve damage is not a problem - I just have a couple of sceptical patients who want " proof " prior to treatment - one in particular is a 80 yr old rancher who wants to get back to roping and wrangling! Kayte Chinese Medicine , " " <@e...> wrote: > Hi Kayte, > > > Are we inferring (or should we?) a connection between acupuncture > > and PEMF and if so is this only for acupuncture with e-stim? > > IMO, acupoints can be activated by many different stimuli: > needling, guasha, electro-AP, magnets, Dermajet, TENS, e-stim, > ultrasound, colour, laser, massage, heat, cold, etc, etc. > > IMO, the POINTS are more important than the STIMULUS applied. > PEMF is only one stimulus, but simple needling could be as > effective, IMO. > > > I have a number of prospective patients with post surgical (carpal > > tunnel in the main) numbness and tingling due to nerve damage - > > this is 12 months post surgery and no improvement. Kayte > > IMO, simple AP at points along the affected channel + related > points (as in Richard Tan's method, could be useful here. Treat as > for peripheral (sensory) nerve paralysis. > > > Best regards, > > Email: <@e...> > > WORK : Teagasc Research Management, Sandymount Ave., Dublin 4, Ireland > Mobile: 353-; [in the Republic: 0] > > HOME : 1 Esker Lawns, Lucan, Dublin, Ireland > Tel : 353-; [in the Republic: 0] > WWW : http://homepage.eircom.net/~progers/searchap.htm Quote Link to comment Share on other sites More sharing options...
Guest guest Posted April 20, 2004 Report Share Posted April 20, 2004 Hi Kayte! Have you been e-stimming them? At 09:29 AM 4/19/2004, you wrote: >Are we inferring (or should we?) a connection between acupuncture and >PEMF and if so is this only for acupuncture with e-stim? > >I have a number of prospective patients with post surgical (carpal >tunnel in the main) numbness and tingling due to nerve damage - this >is 12 months post surgery and no improvement. Regards, Pete Quote Link to comment Share on other sites More sharing options...
Guest guest Posted April 20, 2004 Report Share Posted April 20, 2004 Hi Phil! Don't know where the paragraphs start and end. Maybe it is your email client? Oh, maybe I am just old-fashioned . . . At 10:29 AM 4/19/2004, you wrote: >Hi Pete > > > Hi Phil! Just curious, does your keyboard have a " tab " key? An > > " enter " key? > >Yes, I have standard keyboard. > > > If you do have these available to you, you *could* use them to > > > clarify your postings. Just a thought. At 02:25 PM 4/18/2004, you > > wrote: J.P. Shah,1* P.Midkiff,1 P.C. Brandt,2 and B.F. Sisken1* > > Bioelectromagnetics 22:267-271 (2001) Growth and Differentiation > > of PC6 Cells: the Effects of Pulsed Electromagnetic Fields > > (PEMF) 1Center for Biomedical Engineering and Department > > ofAnatomy and Neurobiology, 2Department of Biochemistry, > > University of ... Regards, Pete > >How were my postings not clear? Regards, Pete Quote Link to comment Share on other sites More sharing options...
Guest guest Posted April 20, 2004 Report Share Posted April 20, 2004 Hi Kayte! Tell the ranch guy to send you a friend in a similar condition who will give you the benefit of the doubt for ten treatments and he can judge by how his friend does. Tell him you can't give him testimonials any more because of HIPAA, so this is the only way he can find out, unless he wants to give you the benefit of the doubt for ten treatments himself - which he may do if you put it like this. At 06:34 PM 4/19/2004, you wrote: >Phil - IMO treating the nerve damage is not a problem - I just have a >couple of sceptical patients who want " proof " prior to treatment - >one in particular is a 80 yr old rancher who wants to get back to >roping and wrangling! > >Kayte Regards, Pete Quote Link to comment Share on other sites More sharing options...
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