Extraction Methods for Peperine

[Guest guest]

Just FYI. Some surprisingly easy methods for extracting Peperine from black pepper from http://www.curevents.com/vb/showthread.php?t=25598.

- Steve

 

" Black pepper, by itself, is not a very efficient way to increase the uptake of curcumin. Piperine makes up only about 3% mass (dry) of the pepper. The breakdown of the pepper material will also cause a lag in piperine availability. Extraction is neccessary.

 

I have a great deal of experience extracting piperine using househould items and have 27g of pure yellow crystals sitting on my desk at this moment. Here is how I do it.

 

Microwave(*1) ~100g of crushed black pepper spread out on a plate; make sure you have a small glass of water also in the microwave to prevent feedback to the magnetron. Shake this microwaved pepper in a mason jar with either denatured alcohol from a harware store or anhydrous isopropyl alcohol(*2) (IPA, rubbing alcohol) for about 10 minutes.

 

You now need to filter out the solids from this mix. I usually make a two stage filter out of the top half of a 1 liter water bottle, a generic cooking funnel, and a few coffee filters.

 

http://img468.imageshack.us/img468/5346/filtersetup1rt.gif

 

Once this finishes filtering you can then evaporate the solvents off the piperine by pouring the solution into a pan with a large surface area and very gently heating it (I sometimes just let it sit without heat for a day or so when lazy). This should leave you with a sticky yellow crystaline substance with bits of brown gel mixed in (this brown is chavacine, responsible for the smell of pepper). Scrape this mix up and disolve in acetone, filter again, then recrystalize by either evaporating off the solvent or dumping the solution into a liter or two of ice cold water.

 

This should leave you with fairly pure piperine.

 

(1) Microwaving black pepper in a non-polar solvent (or by itself) will tend to cause cell rupture allowing for easier extraction of piperine. http://rapidshare.de6610631/Microwave_assisted_extraction_of_piperine.pdf.html

 

(2) You can make dry IPA fairly easily. Buy epsom salts from a grocery store and heat in the oven for ~15 minutes to drive off moisture. Put an appreciable amount of now dry epsom salts into the rubbing alcohol and shake like mad for a minute. Filter the mix and you should have pure IPA without water. It's best to start with 91% IPA if possible but 70% will do. "

[Guest guest]

eeeeee. NO, do not microwave anything!!! Toxic toxic toxic.Just cooking with black pepper causing oxidation. The oxidized byproduct is very irritating to the prostate. Anyone with an enlarge prostate should never eat cooked black pepper.Dr. Goebel--- On Tue, 9/29/09, Norton, Steve <stephen.norton wrote:Norton, Steve <stephen.norton Extraction Methods for Peperineoleander soup Date: Tuesday, September 29, 2009, 5:21 PM

 

 

 

 

 

Just FYI. Some surprisingly easy methods for extracting Peperine from black pepper from http://www.curevent s.com/vb/ showthread. php?t=25598.

- Steve

 

"Black pepper, by itself, is not a very efficient way to increase the uptake of curcumin. Piperine makes up only about 3% mass (dry) of the pepper. The breakdown of the pepper material will also cause a lag in piperine availability. Extraction is neccessary.

 

I have a great deal of experience extracting piperine using househould items and have 27g of pure yellow crystals sitting on my desk at this moment. Here is how I do it.

 

Microwave(*1) ~100g of crushed black pepper spread out on a plate; make sure you have a small glass of water also in the microwave to prevent feedback to the magnetron. Shake this microwaved pepper in a mason jar with either denatured alcohol from a harware store or anhydrous isopropyl alcohol(*2) (IPA, rubbing alcohol) for about 10 minutes.

 

You now need to filter out the solids from this mix. I usually make a two stage filter out of the top half of a 1 liter water bottle, a generic cooking funnel, and a few coffee filters.

 

http://img468. imageshack. us/img468/ 5346/filtersetup 1rt.gif

 

Once this finishes filtering you can then evaporate the solvents off the piperine by pouring the solution into a pan with a large surface area and very gently heating it (I sometimes just let it sit without heat for a day or so when lazy). This should leave you with a sticky yellow crystaline substance with bits of brown gel mixed in (this brown is chavacine, responsible for the smell of pepper). Scrape this mix up and disolve in acetone, filter again, then recrystalize by either evaporating off the solvent or dumping the solution into a liter or two of ice cold water.

 

This should leave you with fairly pure piperine.

 

(1) Microwaving black pepper in a non-polar solvent (or by itself) will tend to cause cell rupture allowing for easier extraction of piperine. http://rapidshare. de 6610631/Microwav e_assisted_ extraction_ of_piperine. pdf.html

 

(2) You can make dry IPA fairly easily. Buy epsom salts from a grocery store and heat in the oven for ~15 minutes to drive off moisture. Put an appreciable amount of now dry epsom salts into the rubbing alcohol and shake like mad for a minute. Filter the mix and you should have pure IPA without water. It's best to start with 91% IPA if possible but 70% will do."

[Guest guest]

I am not arguing with your warning about use of a microwave although I am less worried about microwaves in general. However, I would like to point out that you do not consume the whole cooked peppercorn. The microwaves rupture the cells of the pepper allowing you access to the oils within the pepper. From which you extract only the peperine component. I would guess that you could grind and/or crush the pepper instead of using a microwave but you might get a reduced amount of peperine extracted. I do not think this would cause any more irritation of the prostrate than any commercially extracted peperine.

- Steve

 

 

oleander soup oleander soup On Behalf Of Michael GoebelTuesday, September 29, 2009 3:27 PMoleander soup Subject: Re: Extraction Methods for Peperine

 

eeeeee. NO, do not microwave anything!!! Toxic toxic toxic.Just cooking with black pepper causing oxidation. The oxidized byproduct is very irritating to the prostate. Anyone with an enlarge prostate should never eat cooked black pepper.Dr. Goebel--- On Tue, 9/29/09, Norton, Steve <stephen.norton (AT) ngc (DOT) com> wrote:

Norton, Steve <stephen.norton (AT) ngc (DOT) com> Extraction Methods for Peperineoleander soup Date: Tuesday, September 29, 2009, 5:21 PM

 

Just FYI. Some surprisingly easy methods for extracting Peperine from black pepper from http://www.curevent s.com/vb/ showthread. php?t=25598.

- Steve

"Black pepper, by itself, is not a very efficient way to increase the uptake of curcumin. Piperine makes up only about 3% mass (dry) of the pepper. The breakdown of the pepper material will also cause a lag in piperine availability. Extraction is neccessary.I have a great deal of experience extracting piperine using househould items and have 27g of pure yellow crystals sitting on my desk at this moment. Here is how I do it.Microwave(*1) ~100g of crushed black pepper spread out on a plate; make sure you have a small glass of water also in the microwave to prevent feedback to the magnetron. Shake this microwaved pepper in a mason jar with either denatured alcohol from a harware store or anhydrous isopropyl alcohol(*2) (IPA, rubbing alcohol) for about 10 minutes.You now need to filter out the solids from this mix. I usually make a two stage filter out of the top half of a 1 liter water bottle, a generic cooking funnel, and a few coffee filters.http://img468. imageshack. us/img468/ 5346/filtersetup 1rt.gifOnce this finishes filtering you can then evaporate the solvents off the piperine by pouring the solution into a pan with a large surface area and very gently heating it (I sometimes just let it sit without heat for a day or so when lazy). This should leave you with a sticky yellow crystaline substance with bits of brown gel mixed in (this brown is chavacine, responsible for the smell of pepper). Scrape this mix up and disolve in acetone, filter again, then recrystalize by either evaporating off the solvent or dumping the solution into a liter or two of ice cold water.This should leave you with fairly pure piperine.(1) Microwaving black pepper in a non-polar solvent (or by itself) will tend to cause cell rupture allowing for easier extraction of piperine. http://rapidshare. de 6610631/Microwav e_assisted_ extraction_ of_piperine. pdf.html(2) You can make dry IPA fairly easily. Buy epsom salts from a grocery store and heat in the oven for ~15 minutes to drive off moisture. Put an appreciable amount of now dry epsom salts into the rubbing alcohol and shake like mad for a minute. Filter the mix and you should have pure IPA without water. It's best to start with 91% IPA if possible but 70% will do."

[Guest guest]

A Sohlex (sp?) extractor is the preferred method -- low, light solvent, that you can evaporate with a vacuum pump.Dr.. G--- On Tue, 9/29/09, Norton, Steve <stephen.norton wrote:Norton, Steve <stephen.nortonRE: Extraction Methods for Peperineoleander soup Date: Tuesday, September 29, 2009, 5:45 PM

 

 

 

I am not arguing with your warning about use of a microwave although I am less worried about microwaves in general. However, I would like to point out that you do not consume the whole cooked peppercorn. The microwaves rupture the cells of the pepper allowing you access to the oils within the pepper. From which you extract only the peperine component. I would guess that you could grind and/or crush the pepper instead of using a microwave but you might get a reduced amount of peperine extracted. I do not think this would cause any more irritation of the prostrate than any commercially extracted peperine.

- Steve

 

 

oleander soup [oleandersou p ] On Behalf Of Michael GoebelTuesday, September 29, 2009 3:27 PMoleander soupRe: Extraction Methods for Peperine

 

eeeeee. NO, do not microwave anything!!! Toxic toxic toxic.Just cooking with black pepper causing oxidation. The oxidized byproduct is very irritating to the prostate. Anyone with an enlarge prostate should never eat cooked black pepper.Dr. Goebel--- On Tue, 9/29/09, Norton, Steve <stephen.norton@ ngc.com> wrote:

Norton, Steve <stephen.norton@ ngc.com> Extraction Methods for Peperineoleander soupTuesday, September 29, 2009, 5:21 PM

 

Just FYI. Some surprisingly easy methods for extracting Peperine from black pepper from http://www.curevent s.com/vb/ showthread. php?t=25598.

- Steve

"Black pepper, by itself, is not a very efficient way to increase the uptake of curcumin. Piperine makes up only about 3% mass (dry) of the pepper. The breakdown of the pepper material will also cause a lag in piperine availability. Extraction is neccessary.I have a great deal of experience extracting piperine using househould items and have 27g of pure yellow crystals sitting on my desk at this moment. Here is how I do it.Microwave(*1) ~100g of crushed black pepper spread out on a plate; make sure you have a small glass of water also in the microwave to prevent feedback to the magnetron. Shake this microwaved pepper in a mason jar with either denatured alcohol from a harware store or anhydrous isopropyl alcohol(*2) (IPA, rubbing alcohol) for about 10 minutes.You now need to filter out the solids from this mix. I usually make a two stage filter out of the top half of a 1 liter water bottle, a generic cooking funnel, and a few coffee filters.http://img468. imageshack. us/img468/ 5346/filtersetup 1rt.gifOnce this finishes filtering you can then evaporate the solvents off the piperine by pouring the solution into a pan with a large surface area and very gently heating it (I sometimes just let it sit without heat for a day or so when lazy). This should leave you with a sticky yellow crystaline substance with bits of brown gel mixed in (this brown is chavacine, responsible for the smell of pepper). Scrape this mix up and disolve in acetone, filter again, then recrystalize by either evaporating off the solvent or dumping the solution into a liter or two of ice cold water.This should leave you with fairly pure piperine.(1) Microwaving black pepper in a non-polar solvent (or by itself) will tend to cause cell rupture allowing for easier extraction of piperine. http://rapidshare. de 6610631/Microwav e_assisted_ extraction_ of_piperine. pdf.html(2) You can make dry IPA fairly easily. Buy epsom salts from a grocery store and heat in the oven for ~15 minutes to drive off moisture. Put an appreciable amount of now dry epsom salts into the rubbing alcohol and shake like mad for a minute. Filter the mix and you should have pure IPA without water. It's best to start with 91% IPA if possible but 70% will do."