Inhibitory effect of extract of fungi of Huaier on hepatocellular carcinoma cell

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J Huazhong Univ Sci Technolog Med Sci. 2009 Apr;29(2):198-201. Epub 2009 Apr 28.

Inhibitory effect of extract of fungi of Huaier on hepatocellular carcinoma

cells.

Ren J, Zheng C, Feng G, Liang H, Xia X, Fang J, Duan X, Zhao H.

 

Department of Radiology, Union Hospital, Tongji Medical College, Huazhong

University of Science and Technology, Wuhan, 430022, China,

renjianzhuang.

 

from:http://www.ncbi.nlm.nih.gov/pubmed/19399404?ordinalpos=1 & itool=EntrezSystem\

2.PEntrez.Pubmed.Pubmed_ResultsPanel.Pubmed_Default

ReportPanel.Pubmed_RVDocSum

 

This study investigated the inhibitory effect of the extract of fungi of

Huaier (EFH) on the growth of hepatocellular carcinoma (HCC) cells. Hep-G2

cells, a human HCC cell line, were cultured in DMEM containing 10% fetal bovine

serum and treated with EFH of different concentrations (1, 2, 4, 8 mg/mL) for

24, 48 and 72 h respectively. The apoptosis rate of the cells was flow

cytometrically measured. Thirty-six tumor-bearing New Zealand rabbits were

randomly divided into 3 groups: group A (control group), in which the rabbits

were infused with 0.2 mL/kg normal saline via the hepatic artery; group B

(transhepatic artery chemoembolization [TACE] group), in which the rabbits were

given lipiodol at 0.2 mL/kg plus MMC at 0.5 mg/kg via the hepatic artery; group

C (TACE + EFH group ), in which EFH (500 mg/kg) were orally administered after

TACE. Two weeks after TACE, the rabbits were sacrificed and the implanted tumors

were sampled. The tumor volume and the necrosis rate were determined. The tumor

tissues were immunohistochemically detected for the expressions of factor VIII,

VEGF, P53, Bax and Bcl-2. The microvessel density (MVD) was calculated by

counting the factor VIII-positive endothelial cells. Our results showed that

after treatment with EFH, the apoptosis rate of Hep-G2 cells was enhanced in a

concentration- and time-dependent manner. Two weeks after the treatment, the

average tumor volume, the necrosis rate and the growth rate of the implanted

tumor in group C were significantly different from those in groups A and B

(P<0.05). MVD and VEGF expressions were significantly decreased in the group C

when compared with those in groups B (P<0.05 for all). The Bax expression was

weakest in group A and strongest in group C. The expressions of P53 and Bcl-2

were minimal in group C and maximal in group A. There were significant

differences in the expressions of P53, Bax and Bcl-2 among the 3 groups (P<0.05

for all) and there was significant difference between group B and group C

(P<0.05). It was concluded that EFH could suppress not only the growth of HCC

cells but also tumor angiogenesis and it can induce the apoptosis of HCC cells.

EFH serves as an alternative for the treatment of HCC.

 

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