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Lignans: Beyond Flaxseed Oil JoAnn Guest Jun 19, 2005 13:08 PDT

 

Dallas Clouatre, Ph.D.

http://www.willner.com/article.aspx?artid=58

 

Flaxseed is one of the world's oldest foods. It was consumed by the

ancient Babylonians more than 5,000 years ago and was prized by the

Greeks and Romans for its health-promoting benefits. The seeds

supply both essential fatty acids and fiber, much of it soluble like

that found in fruit and oat bran. Recent research has brought to

light yet more benefits from components contained within flaxseed

fiber known as lignans.

 

Many health-conscious shoppers are familiar with flax oil.

Approximately 41 percent of flaxseed consists of fatty acids, of

which about 70 percent is polyunsaturated. There is a high ratio of

alpha-linolenic acid (an omega-3 fatty acid) to linoleic acid (an

omega-6 fatty acid). More than half the fat in flaxseed is of the

omega-3 fatty acid type.

Both alpha-linolenic acid and linoleic acid are essential because

the body cannot manufacture them from any other substances, yet they

are the parent compounds for many physiologically active substances,

such as eicosanoids.

 

The average American's diet supplies a ratio of the two which is

roughly 1:20 in favor of omega-6, whereas an ideal ratio of omega-3

to omega-6 fatty acids is between 1:1 and 1:3. Therefore, eating

flaxseed or flaxseed oil can help to balance the ratio of these

fatty acids found in the normal diet. Omega-3 fatty acids protect

against coronary heart disease, stroke, hypertension, inflammation,

autoimmune disorders and a number of cancers.

 

What Are Flax Lignans?

Several years ago, I first ran across lignans, another component

found in flaxseed that helps to defend against sex-based cancers in

both women and men. (See The Prostate Miracle, Kennsington Books,

2000.) Lignans are a group of sugar-like molecules that are found

in plants, especially the seeds.

 

Lignans should not be confused with lignin, which is an insoluble

fiber that is structurally related. Indeed, in the plant world,

lignans might be said to be the building blocks for lignin.

 

Lignans are especially abundant in flaxseed, with some authorities

estimating that flax contains from 75 to 800 times as much of these

items as do other food sources. Flax lignans form fibrous chains

that effectively bind toxins found in the gastrointestinal tract.

 

However, flax lignans are far more than just fiber. Lignans are

phytoestrogens, that is, plant compounds which weakly resemble

estrogens, the female sex hormones produced in the human body in

both women and men. Other such phytoestrogenic compounds included

isoflavones, coumestans, flavonoids and phytosterols.

 

Phytoestrogens can compete for receptor sites with the body's own

estrogen when estrogen levels are high or supplement estrogenic

activity when estrogen levels are low. Just as significantly, they

complete with what are known as xenoestrogens. Xenoestrogens are

harmful estrogen-like molecules from the environment, such as are

found in pesticides, solvents, adhesives, soap emulsifiers,

plastics, PCB's, etc.

 

One reason that eating fruits, vegetables and legumes promotes

health and reduces the risk of cancer is that phytoestrogens found

in these foods help to protect us from toxins that we have added to

the environment.

 

Flax is particularly rich in the lignan secoisolariciresinol

diglycoside (SDG), and it also contains small amounts of the lignans

matairesinol, pinoresinol and isolariciresinol.

 

Bacteria in the digestive tract act on some of these lignans and

convert them into yet more potent substances.

 

The lignans SDG, matairesinol and pinoresinol in flax are converted

in the colon of humans and other animals to the mammalian lignans

enterodiol and enterolactone. Enterodiol and enterolactone are

called mammalian lignans because they are produced by bacteria in

the mammalian colon, but are not found in plants. After a

circuitous route through the body, these flax lignan metabolites are

removed by the kidneys.

 

The Benefits of Lignans

Although flax lignans are not as powerful as the body's own

estrogens, they still can exert important effects. In general, flax

lignans are balancing to estrogen metabolism.

 

If the level of estrogens in the blood is high, such as in some

women who suffer from premenstrual syndrome, then lignans will tend

to reduce the body's response.

 

If the level of estrogens in the blood is low, such as in women

after menopause, then lignans will tend to increase the body's

response.

 

Hence, lignans can act either as phytoestrogens (weak estrogens)

or as antiestrogens, depending on the circumstances. The major

benefits of flax lignans that have been researched thus far are in

these areas: protection against breast, prostate and colon cancers;

improvements in cardiovascular health; reduction in menopause

symptoms; antioxidant protection; support for bone health.

 

At this point in time, more than 100 research studies have been

published on flax lignans. Many of these concern the role of

lignans is preventing or ameliorating cancers. As already indicated,

lignans can act either as weak estrogens or as antiestrogens. The

impact on sex hormone sensitive cancers, such as breast and prostate

cancers, may be significant.

 

Flax lignans (after their transformation in the gut to mammalian

lignans) stimulate the production by the liver of sex hormone-

binding globulin (SHBG). This increases the clearance of sex

hormones from the body and may have other effects.

 

By displacing estrogen being carried by SHBG, lignans, moreover,

can reduce the level of estrogen carried to the tissues.

 

Lignans, furthermore, decrease the activity of an enzyme known as

estrogen synthetase (aromatase), which can increase estrogen

production undesirably from fat cells and other sources in men as

well as women.

 

Animal studies and human population studies support the protective

role of lignans against hormone-sensitive cancers. As of yet,

however, clinical trials testing only purified lignans remain rare

and much information must be extrapolated from studies using

flaxseed or reduced-oil flaxseed.

 

In a group of postmenopausal women, consumption of 10 grams of

ground flax daily for seven weeks significantly increased the

urinary excretion of 2-hydroxyestrogen, a metabolite of estrogen

that is considered to protect against breast cancer.

 

In another preliminary study, this one involving 39 women with

newly-diagnosed breast tumors, the subjects every day ate a muffin

containing either no flax or 25 grams of flaxseed for just over a

month. The women who ate the flaxseed muffins exhibited reductions

in breast cell proliferation and in the rate of tumor growth. Men,

too can benefit.

 

At least one pilot clinical trial can be found in the medical

literature regarding prostate cancer. The finding was a reduction

in the markers for cancer activity. Interestingly, flax consumption

does not appear to affect testosterone levels in men and, as noted

already, flax lignans inhibit that activity of the aromatase enzyme

that increases estrogen production.

 

There may be other mechanisms at work than the antiestrogenic

factor. Lignans seem to reduce the incidence of colon cancer, which

is nonhormone-sensitive. Research with patients with prostate and

other cancers has shown that there is a dramatic lowering of the

lignan content in the body wastes compared with non-cancerous

individuals.

 

This suggests that lignan compounds may help boost immune response

and the detoxification of the by-products of metabolic processes.

Research also suggests that flax and its lignans may decrease

angiogenesis (the growth of blood vessels that supply oxygen and

nutrients to tumors for growth).

 

Flaxseed as a source of fiber and omega-3 fatty acids already might

be expected to influence cardiovascular health. Now we know that

flax lignans by themselves offer additional benefits. They possess

antioxidant properties and in animal trials have been found to lower

cholesterol and to protect against induced atherosclerosis.

 

Women may have the most to gain in terms of total health benefits.

Because lignans are phytoestrogens, under conditions of low blood

estrogen levels, such as menopause, lignans can weakly activate

estrogen receptors. This action is similar to that found with

isoflavones from various legumes.

 

Flaxseed consumption (40 grams per day for four months) in one

study was as effective as hormone replacement therapy in reducing

menopause symptoms among 25 menopausal women. Conversely, an

antiestrogenic effect (and perhaps an improved rate of estrogen

clearance) would seem to have been demonstrated in a trials with

premenopausal women taking flax seed who exhibited reduced breast

pain and tenderness during the menstrual period.

 

The fact that flax acts to return balance to sex hormone clearance

and receptor responses rather than simply driving reactions in one

or another direction indicates the inherent safety of flax and its

constituent components.

 

Other potential benefits include improvements in bone health and

kidney function. There even is evidence that suggests that lignans

may help to protect against hair loss and acne. Flaxseed and

flaxseed lignans thus promise a remarkable range of benefits to both

women and men.

Finally, it should not be forgotten that flaxseed can be used to

dramatically improve efforts at losing and maintaining proper weight

while promoting good health. Ann Louise Gittleman gives a very nice

program in The Fat Flush Plan (McGraw-Hill, 2002) and I provide

detailed instructions in Chapter 3 of Anti-Fat Nutrients, 4th

edition (Basic Health Publications, 2004).

 

Differences In Sources of Flaxseed and Other Issues

Flaxseed comes in both brown and golden forms. According to the

Flax Council Of Canada website, there is no significant difference

between brown flax seed and golden (yellow) flax seed in terms of

nutrition. Some individuals prefer the taste of one to the other.

Minor differences in oil content and other characteristics exist

among flax varietals, of course. A couple of rounded tablespoonsful

(12 - 15 grams) of ground flaxseed typically will supply about 200

milligrams of all lignan fractions.

 

To put this in context of the studies discussed above, 10 grams of

flax provides approximated 20 milligrams of secoisolariciresinol,

the active fraction of the primary flax lignan secoisolariciresinol

diglycoside (SDG).

 

Studies with ground flaxseed at dosages from 5 to 50 grams per day

thus supplied 10 to 350 milligrams of secoisolariciresinol. This

indicates the inherent safety of flax and its lignans.

 

The only group which perhaps should not supplement with large

amounts of flaxseed or its lignans (flax oil is OK) is pregnant

women or those looking to conceive. No harm has ever been found,

but simple prudence suggests caution in taking any type of

supplement during pregnancy.

 

 

Flax lignans now are available not only from flaxseed itself, but

also from enriched flax oils and from specially concentrated lignan

extracts. Some authorities suggest that flaxseed is best utilized

when the daily diet is supplemented with small amounts of vitamin B6

(perhaps 5 milligrams) and the mineral zinc (15 milligrams).

 

A good intake of flaxseed is 5 to 30 grams per day. If

supplementing with a concentrated source of secoisolariciresinol

diglycoside (SDG), look to increase intake from up to 50 milligrams

per day.

 

 

Dallas Clouatre, Ph.D., is a Jarrow Formulas consultant based in

Santa Monica, California. He is a prominent industry consultant in

the US, Europe, and Asia, and is a sought-after speaker and

spokesperson. He earned his A.B. from Stanford and his Ph.D. in

European Intellectual History from the University of California at

Berkeley. A member of the American College of Nutrition, he is a

regular contributor to various industry publications. He is the

author of numerous books, including FAQ: All About Grapeseed

Extract, SAM-e: The Ultimate Methyl Donor, Anti-Fat Nutrients (3rd

edition), and The Prostrate Cancer Miracle.

 

 

SELECTED REFERENCES

Adlercreutz, H., Fotsis, T., Heikkinen, R., et al. " Excretion of the

lignans enterolactone and enterodiol and of equol in omnivorous and

vegetarian postmenopausal women and in women with breast cancer. "

Lancet (1982). Vol. 2 pp. 1295-9.

Adlercreutz, H., Bannwart, C., Wahala ,K., et al. " Inhibition of

human aromatase by mammalian lignans and isoflavonoid

phytoestrogens. " J Steroid Biochem Mol Biol. (1993). Vol. 44 pp. 147-

53.

Adlercreutz, H., Hockerstedt, K., Bannwart, C., et al. " Effect of

dietary components, including lignans and phytoestrogens, on

enterohepatic circulation and liver metabolism of estrogens and on

sex hormone binding globulin (SHBG). " J Steroid Biochem. (1987).

Vol. 27 pp. 1135-44.

Arjmandi, B. H., Juma, S., Lucas, E. A., et al. " Flaxseed

supplementation positively influence bone metabolism in

postmenopausal women. " JANA (1998). Vol. 1 pp. 27-32.

Dabrosin, C., Chen, J., Wang, L., Thompson, L. U. " Flaxseed inhibits

metastasis and decreases extracellular vascular endothelial growth

factor in human breast cancer xenografts. " Cancer Letters (2000).

Vol. 185 pp. 31-7.

Demark-Wahnefried, W., Price, D. T., Polascik, T. J., Robertson, C.

N., et al. " Pilot study of dietary fat restriction and flaxseed

supplementation in men with prostate cancer before surgery:

exploring the effects on hormonal levels, prostate-specific antigen,

and histopathologic features. " Urology (2001). Vol. 58 pp. 47-52.

Denis, L., Morton, M. S., Griffiths, K. " Diet and its preventive

role in prostatic disease, " Eur Urol. (1999). Vol. 35 pp. 377-87.

Evans, B. A. J., Griffiths, K., Morton, M. S. " Inhibition of 5 alpha-

reductase in genital skin fibroblasts and prostate tissue by dietary

lignans and isoflavonoids. " Journal of Endocrinology (1995). Vol.

147 pp. 295-302.

Haggans, C. J., Hutchins, A. M., Olson, B. A., et al. " Effect of

flaxseed consumption on urinary estrogen metabolites in

postmenopausal women. " Nutr Cancer (1999). Vol. 33 pp. 188-95.

Hagans, C. J., Travelli, E. J., Thomas, W., et al. " The effect of

flaxseed and wheat bran consumption on urinary estrogen metabolites

in premenopausal women. " Cancer Epidemiol Biomarkers Prev. (2000).

Vol. 9 pp. 719-25.

Lemay, A., Dodin, S., Kadri, N., et al. " Flaxseed dietary supplement

versus hormone replacement therapy in hypercholesterolemic

menopausal women. " Obstet Gynecol. (2002). Vol. 100 pp. 495-504.

Lin, X., Gingrich, J. R., Bao, W., et al. " Effect of flaxseed

supplementation on prostatic carcinoma in transgenic mice. " Urology

(2002). Vol. 60 pp. 919-24.

Lin, X., Switzer, B. R., Demark-Wahnefried, W. " Effect of mammalian

lignans on the growth of prostate cancer cell lines. " Anticancer

Research (2001). Vol. 21 pp. 3995 -4000.

Lucas, E. A., Wild, R. D., Hammond, L. J., et al. " Flaxseed improves

lipid profile without alterine biomarkers of bone metabolism in

postmenopausal women. " J Clin Endocrinol Metab. (2002). Vol. 87 pp.

1527-32.

Morton, M. S., Matos-Ferreira, A., Abranches-Monteiro, L., et

al. " Measurement and metabolism of isoflavonoids and lignans in the

human male. " Cancer Letters (1997). Vol. 114 pp. 145-51.

Pietinen, P., Stumpf, K., Mannisto, S., et al. " Serum enterolactone

and risk of breast cancer: a case-control study in eastern Finland. "

Cancer Epidemiol Biomarkers Prev. (2001). Vol. 10 pp. 339-44.

Prasad, K. " Flaxseed and Atherosclerosis " In: Thompson, L. U. and

Cunnane, S. C., ed. Flaxseed in Human Nutrition, 2nd ed. Champaign,

IL: AOCS Press (2003). pp. 260-73.

Prasad, K. " Antioxidant Activity of Secoisolariciresinol Diglycoside-

derived Metabolites, Secoisolariciresinol, Enterodiol and

Enterolactone, " International Journal of Angiology (2000). Vol. 9

pp. 220-5.

Thompson, L. U., Li, T., Chen, J., Goss, P. E. " Biological effects

of dietary flaxseed in patients with breast cancer " (abstract).

Breast Cancer Res Treat. (2000). Vol. 64 p. 50.

Thompson, L. U. " Experimental studies on lignans and cancer. "

Bailliere's Clinical Endocrin Metab. (1998).

Vol. 12 p. 691-705.

 

 

A Matter of Perspective ...

by Dr. Michael Murray

 

 

It amazes me that adverse reactions to prescription drugs are

estimated to kill over 106,000 Americans every year, yet all it

seems that the media is interested in is inaccurately portraying the

dietary supplement industry as dangerous and " unregulated. " I think

a little perspective is in order.

 

Consumer Reports " Dirty Dozen "

 

Recently, Consumer Reports came up with what they referred to as

the " Dirty Dozen. " Actually, this report is actually the stimulus of

this editorial. While I agree that some of the compounds listed are

potentially harmful, when you take a look at the list as a whole it

is laughable compared to the dangers of even as something as simple

as aspirin.

 

What am I talking about? It is a well established fact that each

year the use of aspirin and other NSAIDs (Non-Steroidal Anti-

Inflammatory Drugs) accounts for an estimated 7,600 deaths and

76,000 hospitalizations in the United States. " NSAIDs include

aspirin, ibuprofen, naproxen, diclofenac, ketoprofen, and

tiaprofenic acid.

 

 

While I agree that androstenedione (or andro, for short) is

certainly not a dietary supplement and that aristolochic acid and

pennyroyal oil are definitely hazardous, most of the health food

stores that I have been in do not carry these products or others on

the Consumer Reports list.

For example, the natural product industry has been aware of the

dangers of the pyrrolizidine alkaloids found in comfrey for almost

20 years. In fact, I haven't seen a comfrey product designed for

oral use containing these compounds in a health food store in the

last 15 years or so. Now, I could be wrong and there could be some

loose comfrey being sold as a tea out there, but my point is that is

comfrey really a public health hazard? My point here is if you

really take a look at some of the other members of the 'dirty

dozen, " you will see that they pose little risk simply because they

are not widely available.

 

 

The media keeps harping on the dangers of ephedra, but was ephedra

really all that bad? My feeling is that it was the abuse of ephedra

that was the primary issue. Used responsibly at appropriate dosages

there is no question that ephedra is a safe and effective natural

product.

Granted, there was the tendency of abuse because of the typical

American belief that if a little is good, a lot is even better. But,

again let's try to keep things in perspective.

 

In the worst case scenario, over the last 20 years ephedra was

linked to approximately 150 deaths (virtually all of which were

related to excessive dosage or abuse). In contrast, approximately

2,000,000 people in the United States died from adverse drug

reactions including over 140,000 deaths caused by aspirin and other

NSAIDs.

 

 

Taking anything by mouth, whether it is a food, drug, or supplement

requires some personal responsibility. But, the relative risk of

danger from taking a nutritional supplement or herbal product is

substantially less than that seen with prescription and over-the-

counter drugs. That fact is very clear.

 

 

Table - Consumer Reports Dirty Dozen

Androstenedione (or andro, for short)

Aristolochic acid

Bitter orange

Chaparral

Comfrey

Germander

Kava (or kava kava)

Lobelia

Organ/glandular extracts

Pennyroyal oil

Skullcap

Yohimbe

 

 

 

 

Adverse Drug Reactions (ADRs)

 

 

It has been difficult to get a true accounting of the scope of the

problem with adverse drug reactions. Why? It is because fewer than

10% of all such effects are ever reported. Reporting ADRs to the FDA

is a voluntary system even if the drug caused significant harm or

even death.

 

The data that does exist on the scope of the problem with ADRs is

staggering. For example, based upon detailed analysis and

projections presented in the most respected medical journals

including the Journal of the American Medical Association it is

estimated that 6.7% of hospitalized patients have a serious adverse

drug reaction with a fatality rate of 0.32%.

 

This translates to more than 2,216,000 serious ADRs in hospitalized

patients, causing over 106,000 deaths annually.1 These sobering

statistics do not include the number of ADRs that occur outside the

hospital or the estimated 350,000 ADRs that occur in U.S. nursing

homes each year.2

 

 

If we put these statistics into monetary figures it is estimated

that the cost of drug-related illness and mortality is likely now

over $200 billion annually.3 To put this dollar amount into

perspective this amount is more than the total cost of treating

diabetes or heart disease in the United States.

 

 

The obvious question is " Why are there so many ADRs? " There are many

reasons. Here are just a few.

 

 

First, more people are being placed on drugs than ever before. For

example, roughly 4 billion prescriptions were filled last year. That

is about 12 prescriptions for every person in the United States.

Second, more and more people are being placed on a combination of

drugs - many times to deal with side effects caused by some of the

other drugs. The rate of ADRs increases exponentially after a

patient is on 4 or more medications.4

 

Third, many of the newer drugs are more dangerous than older drugs.

The FDA has approved many of these drugs without complete safety

data. Drug companies can now pay a fee to speed up approval. Of the

548 new drugs approved by the FDA from 1975-1999, 56 of these (10%)

were given a new black box warning because of severe ADRs or were

withdrawn from the market because of reports of deaths.

 

Lastly, the majority of health complaints patients see doctors for

owe their origin to dietary and lifestyle factors. Trying to treat

the symptoms with a drug (a biochemical band-aid) fails to address

the underlying cause and leads to side effects as a result.

 

One of the big reasons why more people are relying on drugs more

than ever before is direct to consumer advertising by drug

companies. Prior to 1997 drug companies were prohibited from

marketing their product directly to consumers.

 

If you watch the evening news or soap operas, or read magazines like

Time and Newsweek or read newspapers, it is clear that ads for

prescription and over-the-counter drugs dominate advertising.

 

In fact, the marketing budget alone of the drug companies is 50%

greater than the total retail sales for every vitamin, mineral, or

herbal product sold in the United States through any channel of

trade.

 

Direct to consumer advertising is estimated to have increased the

number of prescriptions last year by nearly 30 million. As an

example of the power of direct to consumer advertising, the sexual

potency drug, Viagra, can probably serve as the poster child. Within

a few months of its introduction, several million men began taking

Viagra, and many serious side effects, including fatalities,

suddenly appeared.

 

 

The FDA has no idea on how deep the problem with ADRs runs relying

on their current reporting system. For example, the FDA receives an

average of 80 reports each year about adverse reactions caused by

the drug digoxin. This relatively small number of reports seems to

indicate that digoxin was not a big problem.

However a systematic survey of Medicare records revealed 202,211

hospitalizations for adverse reactions to digoxin during a seven-

year period.

 

 

The Power of the Drug Companies

 

 

Two years ago an entire issue of the Journal of the American Medical

Association (JAMA 2002 vol. 287 #21) was devoted to looking at some

major problems with the relationship between drug companies,

physicians, the FDA, and medical journals. Here are just of few of

the findings:

 

 

More than half of the experts hired to advise the government on the

safety and effectiveness of drugs have financial relationships with

drug companies that will be helped or hurt by their decisions.

 

Federal law generally prohibits the FDA from using experts with

financial conflicts of interest, but the FDA has waived the

restriction more than 800 times from January 1998 to June 2000.

 

Approximately 85% of authors of " Clinical Practice Guidelines " are

financially dependent upon drug companies.

 

Of 359 studies from JAMA, NEJM, BMJ, AIM, & Lancet only 26 did not

contain misleading statistics.

 

Of new releases on studies receiving drug company financing only 22%

reported the source of funding.

 

Negative findings are suppressed.

One of the conclusions of the editors was that the issues

presented " underscore that the findings presented in the press and

medical journals are not always facts. " I think these issues have

far greater social significance than some of the " non-issues " that

the media have tried to create over the last few years in regards to

natural products. Wouldn't you agree?

 

 

The Bottom Line

 

 

It seems that the FDA seems intent on trying to create a shroud of

doubt on the safety of natural products. The media feeds on this

attempted frenzy and repeatedly states that the dietary supplement

industry is " unregulated. " I don't understand how that statement can

continually pop up in the media when there are well-established

federal regulations governed and enforced by the FDA regarding the

safety and manufacture of dietary supplements.

 

I hope that the media is not being swayed into a bias against the

supplement industry just because a major portion of its advertising

revenue is coming from the drug companies.

 

 

My suggestion to the FDA is that instead of focusing energy on

trying to create issues with the supplement industry, it would serve

us better by developing programs and procedures to better protect us

all from a much more significant danger - adverse drug reactions.

 

In addition, it seems that the FDA should attempt to do a better job

of separating itself from the interests of drug companies and

instead better serve the public's interest.

If that were the case, I think there would be a dramatic move

towards the promotion of the use of natural products to promote

health.

 

 

Key References:

 

 

Pomeranz B, Corey PN. Incidence of adverse drug reactions in

hospitalized patients: A meta-analysis of prospective studies. JAMA

1998;279:1200-1205.

Avorn J, McCormick D, Jain S, Eckler M, et al. Incidence and

preventability of adverse drug events in nursing homes. Am J Med

2000;109(2):87-94.

Johnson JA, Bootman JL. Drug-related morbidity and mortality. A cost-

of-illness model. Arch Intern Med 1995;155(18):1949-1956.

Jacubeit T, Drisch D, Weber E. Risk factors as reflected by an

intensive drug monitoring system. Agents Actions 1990;29: 117-125.

 

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Disclaimer

 

The information provided on this site, or linked sites, is provided

for informational purposes only, and should not be used as a

substitute for advice from your physician or other health care

professional. Product information contained herein has not

necessarily been evaluated or approved by the U.S. Food and Drug

Administration, and is not intended to diagnose, treat, cure or

prevent disease.

 

 

 

 

AIM Barleygreen

" Wisdom of the Past, Food of the Future "

 

http://www.geocities.com/mrsjoguest/Diets.html

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

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