Effect of Vitamin C Supplementation on Oxidative DNA Damage in an Experimental Model of Lead Induced Hypertension

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Effect of Vitamin C Supplementation on Oxidative DNA Damage in an Experimental

Model of Lead Induced Hypertension JoAnn Guest Jun 08, 2005 17:24 PDT

 

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Original Paper

 

Effect of Vitamin C Supplementation on Oxidative DNA Damage in an

Experimental Model of Lead-Induced Hypertension

Jyotika Attria, Veena Dhawana, Safrun Mahmooda, Promila Pandhia, H.K.

Parwanab, Ravinder Natha

 

aDepartments of Experimental Medicine and Biotechnology, and

Pharmacology, Postgraduate Institute of Medical Education and Research,

Chandigarh, and

bPunjab Pollution Control Board, Patiala, India

 

 

Address of Corresponding Author

 

Annals of Nutrition & Metabolism 2003;47:294-301 (DOI:

10.1159/000072402)

 

 

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Key Words

 

Blood pressure

Nitric oxide

Oxidative stress

DNA damage

 

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Abstract

 

Aims: Chronic exposure to lead results in sustained hypertension in

humans and experimental animals. We investigated the possible role of

reactive oxygen species (ROS) and their impact on DNA damage in

lead-induced hypertension.

 

Further the effect of short-term

supplementation of vitamin C is also demonstrated.

 

Methods: Male Wistar

rats were treated with either lead acetate (100 ppm) alone or lead

acetate plus vitamin C (20 mg/rat/day). The control rats were fed

regular rat chow. Blood pressure, antioxidants, total antioxidant status

as measured by ferric-reducing antioxidant power, nitric oxide (NO)

metabolites, malondialdehyde (MDA) and 8-hydroxy 2-deoxyguanosine were

determined after 0, 1, 2 and 3 months.

 

Results: The lead-exposed group

showed a significant rise in blood pressure, lipid peroxidation (MDA)

and a substantial oxidative damage to the DNA. A significant fall in NO

metabolites, total antioxidant levels and ferric-reducing antioxidant

power was also observed in this group.

 

Concomitant administration of

vitamin C ameliorated hypertension, normalized NO levels and abrogated

lipid peroxidation. Also, it completely prevented oxidative damage to

the DNA.

 

Conclusions: These findings point to enhanced ROS-mediated

inactivation and sequestration of NO which can potentially contribute to

hypertension, lipid peroxidation, reduced antioxidant status and

oxidative DNA damage. The beneficial effects of vitamin C on these

parameters support the role of increased ROS activity in the

pathogenesis of these abnormalities in this model.

 

2003 S. Karger AG, Basel

 

 

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Author Contacts

 

Dr. Veena Dhawan, PhD

Department of Experimental Medicine and Biotechnology

Postgraduate Institute of Medical Education and Research

Chandigarh 160012 (India)

Tel. +91 172 567116, Fax +91 172 747403, E-Mail veena-

 

 

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Article Information

 

Received: July 22, 2002

Accepted: February 6, 2003

Number of Print Pages : 8

Number of Figures : 7, Number of Tables : 2, Number of References : 51

 

 

Free Abstract Article (References) Article (PDF 183 KB)

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