central nervous system tissue in meat products

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Madcow

Sun, 10 Apr 2005 10:01:10 -0700 (PDT)

detection of central nervous system

tissue in meat products

 

 

http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve & db=pubmed & dopt=Abstra\

ct & list_uids=15771196 & itool=iconabstr

 

 

: J Food Prot. 2005 Mar;68(3):630-2. Related Articles, Links

 

Comparison of commercially available antibodies for the detection

of central nervous system tissue in meat products by enzyme-linked

immunosorbent assay.

 

Rencova E.

 

Veterinary Research Institute, Hudcova 70, 621 32 Brno, Czech

Republic. rencova

 

An indirect competitive enzyme-linked immunosorbent assay was used

to compare four commercially available monoclonal and polyclonal

antibodies prepared against the markers of central nervous system

tissue: glial fibrillary acidic protein, neurofilaments, and myelin

basic protein. These antibodies were investigated for their usefulness

at the detection of prohibited central nervous system tissue in meat

products to prevent spread of bovine spongiform encephalopathy and its

human form, a new variant of Creutzfeldt-Jakob disease. The detection

limit was 2.5 to 10%. The specificity was tested using extracts of

tissue samples of bovine and porcine brain, spinal cord, lymphatic

node, diaphragm, skeletal muscle, and peripheral nerves. All

antibodies reacted with both bovine and porcine tissue extracts.

 

PMID: 15771196 [PubMed - in process]