Anti-HIV activity of olive leaf extract (OLE)

[Guest guest]

Anti-HIV activity of olive leaf extract (OLE) and modulation of host

cell gene expression by HIV-1 infection and OLE treatment.

 

Lee-Huang S, Zhang L, Huang PL, Chang YT, Huang PL.

 

Department of Biochemistry, New York University School of Medicine,

New York, NY 10016, USA. Sylvia.lee-huang

 

We investigated the antiviral activity of olive leaf extract (OLE)

preparations standardized by liquid chromatography-coupled mass

spectrometry (LC-MS) against HIV-1 infection and replication. We

find that OLE inhibits acute infection and cell-to-cell transmission

of HIV-1 as assayed by syncytia formation using uninfected MT2 cells

co-cultured with HIV-1-infected H9 T lymphocytes. OLE also inhibits

HIV-1 replication as assayed by p24 expression in infected H9 cells.

These anti-HIV effects of OLE are dose dependent, with EC(50)s of

around 0.2 microg/ml. In the effective dose range, no cytotoxicity

on uninfected target cells was detected. The therapeutic index of

OLE is above 5000. To identify viral and host targets for OLE, we

characterized gene expression profiles associated with HIV-1

infection and OLE treatment using cDNA microarrays. HIV-1 infection

modulates the expression patterns of cellular genes involved in

apoptosis, stress, cytokine, protein kinase C, and hedgehog

signaling. HIV-1 infection up-regulates the expression of the heat-

shock proteins hsp27 and hsp90, the DNA damage inducible transcript

1 gadd45, the p53-binding protein mdm2, and the hedgehog signal

protein patched 1, while it down-regulates the expression of the

anti-apoptotic BCL2-associated X protein Bax. Treatment with OLE

reverses many of these HIV-1 infection-associated changes. Treatment

of HIV-1-infected cells with OLE also up-regulates the expression of

the apoptosis inhibitor proteins IAP1 and 2, as well as the calcium

and protein kinase C pathway signaling molecules IL-2, IL-2Ralpha,

and ornithine decarboxylase ODC1.

 

PMID: 12878215 [PubMed - indexed for MEDLINE]

 

http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?

cmd=Retrieve & db=PubMed & list_uids=12878215 & dopt=Abstract

[Guest guest]

, MarthaRolfe@a...

wrote: I notice this says it is dose dependent. I don't understand

what sort of dosages they would be talking about here -- can anyone

explain?

> Thank you very much, Marti

 

Marti,

Referencing the previous article written by Dr. Pivatera (he is

the expert here :-)), it mentions the optimal dosages for HIV. Many

of the benefits are derived from the accompanying flavonoids.

 

Higher doses may be used as well, however a common sense approach

would indicate starting with the minimal amounts recommended and

progressing gradually to a larger doseage.

The most fabulous attribute of this herb is that there is absolutely

no 'side effects' other than the usual 'die-off' symptoms. Another

very good reason to start with minimal amounts in my estimation.

 

As I say, there isn't any toxicity from this herb, however the only

drawback would be adjusting the dosages in case of extremely

severe 'die-off' symptoms.

The important thing is to 'listen' to your body and give it what it

needs and not listen to the negative vibes coming from orthodox

medicine. These herbs are 100% safe in every way when taken as

directed. Allopathic medicine tries to instill fear regarding their

use. This is so ludicrous. I have taken olive leaf extract for

everything from a common cold or virus to high fevers, infections

and some of the more serious illnesses.

There are no ill effects other than minimal passing discomfort

from 'die-off'. This is a normal detox effect! Experimentation is

the key. Everyone is biologically different. These are only general

guidelines put in place by Dr. Privatera who by the way uses this in

his clinic on a daily basis. I can assure you that this doctor has

all the right credentials and has studied this wonderful extract for

years. This article is the result of many years of research.

Best Regards, JoAnn

 

 

 

Dr. Privatera's Recommended Dosages

---

Olive leaf extract is currently available in the form of 500 mg.

tablets. The routine dosage is one tablet every six hours or four

throughout the day. Take the supplement between meals for best

results.

In the case of bad colds or flu, you can use two tablets every six

hours. For acute infections, some individuals have taken more --

three and even four every six hours -- and reported rapid relief.

 

If you encounter a " die-off " effect, cut back on the number of

tablets you are taking or temporarily discontinue them until the

symptoms subside. See the section

on " die-off effect. "

 

For healthy folks seeking more energy or the prevention benefits of

olive leaf extract, we suggest one or two tablets a day. The younger

and cleaner the body, the more responsive it is to supplements such

as this. When a person becomes older and more toxic, more of the

supplement is required to do the job.