Ascorbic acid inhibits the increase in low-density lipoprotein (LDL) susceptibility to oxidation and the proportion of electronegative LDL induced by intense aerobic exercise.

[Guest guest]

Hi All,

 

In this paper it is shown that aerobic exercise does increase oxidized LDL and

that the use of 1 g of Vit C before the

activity eliminates the normal increase in oxidized LDL which results from the

increased free radical load generated by

the exercise.

 

Note the conclusions.

 

http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve & db=PubMed & list_uids=9\

710684 & dopt=Abstract

Coron Artery Dis 1998;9(5):249-55 Related Articles, Books, LinkOut

Ascorbic acid inhibits the increase in low-density lipoprotein (LDL)

susceptibility to oxidation and the proportion of

electronegative LDL induced by intense aerobic exercise.

Sanchez-Quesada JL, Jorba O, Payes A, Otal C, Serra-Grima R, Gonzalez-Sastre F,

Ordonez-Llanos J.

Servei de Bioquimica, Hospital de la Santa Creu i Sant Pau, Barcelona, Spain.

 

BACKGROUND:

We have previously reported the finding of an acute increment in the

susceptibility of low-density lipoprotein (LDL) to

oxidation and in the proportion of electronegative LDL [LDL(-)] after intense

exercise. We have now studied the effect

of oral supplementation with 1 g ascorbic acid, immediately before a 4-h

athletic race, on the susceptibility of LDL to

oxidation, the proportion of LDL(-), and the alpha-tocopherol and lipid

peroxides content in LDL, in order to inhibit

such deleterious changes, and to confirm the oxidative nature of modifications

of LDL induced by exercise.

 

METHODS:

We studied seven highly trained runners who received a supplement of 1 g

ascorbic acid and a control group of seven who

did not receive the supplement. The susceptibility of LDL to oxidation was

assessed by measurement of conjugated dienes

after CuSO4-induced oxidation, the proportion of LDL(-) was determined by anion

exchange chromatography,

alpha-tocopherol was quantified by reverse-phase high performance liquid

chromatography, and lipid peroxides were

measured by the thiobarbituric acid-reactive substances (TBARS) method.

 

RESULTS:

After exercise, in the control group there was an increase in both the

susceptibility of LDL to oxidation (change in lag

phase from 51.4 +/- 4.7 min to 47.0 +/- 4.6 min, P < 0.05) and the proportion of

LDL(-) (from 11.1 +/- 1.4% to 13.0 +/-

2.2%, P < 0.05), but these did not occur in the ascorbic acid group (change in

lag phase from 49.7 +/- 2.3 min to 50.4

+/- 4.2 min, and in LDL(-) from 9.7 +/- 1.7% to 10.1 +/- 1.7%). No significant

changes in the absolute amount of LDL

alpha-tocopherol were observed after exercise (ascorbic acid group: 6.65 +/-

0.94 mol/mol apoB before the race, 7.13 +/-

0.88 mol/mol apoB after the race; control group: 7.34 +/-0.69 mol/mol apoB

before the race, 7.06 +/- 0.69 mol/mol apoB

after the race), but significant differences were found when increments or

decrements of alpha-tocopherol were tested

(alpha-tocopherol increased 9.9 +/- 11.5% in the ascorbic acid group, and

decreased 0.6 +/- 7.3% in the control group; P

< 0.018). TBARS did not change after exercise.

 

CONCLUSIONS:

We conclude that 1 g ascorbic acid inhibits the increase in LDL susceptibility

to oxidation after exercise, preventing

this acute pro-atherogenic effect. In addition, the observation that LDL(-)

enhancement is prevented by ascorbic acid

supports the hypothesis that at least some of the circulating LDL(-) originates

from oxidative processes.

 

PMID: 9710684 [PubMed - indexed for MEDLINE]

 

========================

Good Health & Long Life,

Greg Watson, http://optimalhealth.cia.com.au gowatson

USDA database (food breakdown) http://www.nal.usda.gov/fnic/foodcomp/

PubMed (research papers) http://www.ncbi.nlm.nih.gov/entrez/query.fcgi

DWIDP (nutrient analysis) http://www.walford.com/dwdemo/dw2b63demo.exe

Patch file for above http://www.walford.com/download/dwidp67u.exe

KIM (omega analysis) http://ods.od.nih.gov/eicosanoids/KIM_Install.exe

[Guest guest]

Greg

Thank you for these fascinating findings. Notice that the paper states that

this increase in oxidized LDL is seen during " intense " aerobic exercise.

But this leaves unclarified, the matter of " level of fitness, " in

determining what constitutes " intense " exercise. I'm guessing that people

in ill health, at low levels of fitness, will probably experience this

increase in LDL oxidation at what appear, to the robustly healthy indivdual,

to be much lower levels of exercise intensity. I suffer from angina, so

that even low levels of aerobic exercise, can be very stressful, and

sometimes even unendurable.

John P.

-

" Greg Watson " <gowatson

" Health coconut-info " <coconut-info >

Saturday, January 19, 2002 1:41 AM

Ascorbic acid inhibits the increase in low-density

lipoprotein (LDL) susceptibility to oxidation and the proportion of

electronegative LDL induced by intense aerobic exercise.

 

 

> Hi All,

>

> In this paper it is shown that aerobic exercise does increase oxidized LDL

and that the use of 1 g of Vit C before the

> activity eliminates the normal increase in oxidized LDL which results from

the increased free radical load generated by

> the exercise.

>

> Note the conclusions.

>

>

http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve & db=PubMed & list_ui

ds=9710684 & dopt=Abstract

> Coron Artery Dis 1998;9(5):249-55 Related Articles, Books, LinkOut

> Ascorbic acid inhibits the increase in low-density lipoprotein (LDL)

susceptibility to oxidation and the proportion of

> electronegative LDL induced by intense aerobic exercise.

> Sanchez-Quesada JL, Jorba O, Payes A, Otal C, Serra-Grima R,

Gonzalez-Sastre F, Ordonez-Llanos J.

> Servei de Bioquimica, Hospital de la Santa Creu i Sant Pau, Barcelona,

Spain.

>

> BACKGROUND:

> We have previously reported the finding of an acute increment in the

susceptibility of low-density lipoprotein (LDL) to

> oxidation and in the proportion of electronegative LDL [LDL(-)] after

intense exercise. We have now studied the effect

> of oral supplementation with 1 g ascorbic acid, immediately before a 4-h

athletic race, on the susceptibility of LDL to

> oxidation, the proportion of LDL(-), and the alpha-tocopherol and lipid

peroxides content in LDL, in order to inhibit

> such deleterious changes, and to confirm the oxidative nature of

modifications of LDL induced by exercise.

>

> METHODS:

> We studied seven highly trained runners who received a supplement of 1 g

ascorbic acid and a control group of seven who

> did not receive the supplement. The susceptibility of LDL to oxidation was

assessed by measurement of conjugated dienes

> after CuSO4-induced oxidation, the proportion of LDL(-) was determined by

anion exchange chromatography,

> alpha-tocopherol was quantified by reverse-phase high performance liquid

chromatography, and lipid peroxides were

> measured by the thiobarbituric acid-reactive substances (TBARS) method.

>

> RESULTS:

> After exercise, in the control group there was an increase in both the

susceptibility of LDL to oxidation (change in lag

> phase from 51.4 +/- 4.7 min to 47.0 +/- 4.6 min, P < 0.05) and the

proportion of LDL(-) (from 11.1 +/- 1.4% to 13.0 +/-

> 2.2%, P < 0.05), but these did not occur in the ascorbic acid group

(change in lag phase from 49.7 +/- 2.3 min to 50.4

> +/- 4.2 min, and in LDL(-) from 9.7 +/- 1.7% to 10.1 +/- 1.7%). No

significant changes in the absolute amount of LDL

> alpha-tocopherol were observed after exercise (ascorbic acid group: 6.65

+/- 0.94 mol/mol apoB before the race, 7.13 +/-

> 0.88 mol/mol apoB after the race; control group: 7.34 +/-0.69 mol/mol apoB

before the race, 7.06 +/- 0.69 mol/mol apoB

> after the race), but significant differences were found when increments or

decrements of alpha-tocopherol were tested

> (alpha-tocopherol increased 9.9 +/- 11.5% in the ascorbic acid group, and

decreased 0.6 +/- 7.3% in the control group; P

> < 0.018). TBARS did not change after exercise.

>

> CONCLUSIONS:

> We conclude that 1 g ascorbic acid inhibits the increase in LDL

susceptibility to oxidation after exercise, preventing

> this acute pro-atherogenic effect. In addition, the observation that

LDL(-) enhancement is prevented by ascorbic acid

> supports the hypothesis that at least some of the circulating LDL(-)

originates from oxidative processes.

>

> PMID: 9710684 [PubMed - indexed for MEDLINE]

>

> ========================

> Good Health & Long Life,

> Greg Watson, http://optimalhealth.cia.com.au gowatson

> USDA database (food breakdown) http://www.nal.usda.gov/fnic/foodcomp/

> PubMed (research papers) http://www.ncbi.nlm.nih.gov/entrez/query.fcgi

> DWIDP (nutrient analysis) http://www.walford.com/dwdemo/dw2b63demo.exe

> Patch file for above http://www.walford.com/download/dwidp67u.exe

> KIM (omega analysis)

http://ods.od.nih.gov/eicosanoids/KIM_Install.exe

>

>

>

>

> Getting well is done one step at a time, day by day, building health

> and well being.

>

> To learn more about the Gettingwell group,

> Subscription and list archives are at:

> Gettingwell

>

>

[Guest guest]

-

" John Polifronio " <counterpnt

 

Sunday, January 20, 2002 12:25 AM

Re: Ascorbic acid inhibits the increase in low-density

lipoprotein (LDL) susceptibility to

oxidation and the proportion of electronegative LDL induced by intense aerobic

exercise.

 

 

> Greg

> Thank you for these fascinating findings. Notice that the paper states that

> this increase in oxidized LDL is seen during " intense " aerobic exercise.

> But this leaves unclarified, the matter of " level of fitness, " in

> determining what constitutes " intense " exercise. I'm guessing that people

> in ill health, at low levels of fitness, will probably experience this

> increase in LDL oxidation at what appear, to the robustly healthy indivdual,

> to be much lower levels of exercise intensity. I suffer from angina, so

> that even low levels of aerobic exercise, can be very stressful, and

> sometimes even unendurable.

 

Hi John,

 

Exercise increases free radical production. The more intense the exercise, the

more free radicals are generated. I now

take both 1 g Vit C and 500 IUs Vit E before by exercises with 1 tablespoon of

VCNO and 2 g Glutamine after to speed

recovery. I never exercise longer than 30 minutes as the catabolic and

pro-aging hormone Cortisol slowly builds up.

========================

Good Health & Long Life,

Greg Watson, http://optimalhealth.cia.com.au gowatson

USDA database (food breakdown) http://www.nal.usda.gov/fnic/foodcomp/

PubMed (research papers) http://www.ncbi.nlm.nih.gov/entrez/query.fcgi

DWIDP (nutrient analysis) http://www.walford.com/dwdemo/dw2b63demo.exe

Patch file for above http://www.walford.com/download/dwidp67u.exe

KIM (omega analysis) http://ods.od.nih.gov/eicosanoids/KIM_Install.exe

[Guest guest]

Evening Greg,

 

>Exercise increases free radical production. The more intense the

>exercise, the more free radicals are generated.

 

So there is no way to avoid this? The best thing to do is take the

addition supplements before the exercise session, right.

 

Still, we have to face a bit of this " free radical generation " , or else

we do no exercise. This must mean there is some

magic amount of exercise that is right for us.

 

Your logic on this issue does fill in the gaps on many things I have

read written by doctors. They however, did not explain the full details

and what should be done to lessen the effect of the free radicals.

 

You also pointed out that when I exercise with oxygen, I make this

worse. Surely, if you try real hard, you can think of some benefit to

doing exercise with oxygen.

 

 

In Johns case.......

 

> > to be much lower levels of exercise intensity. I suffer from angina, so

> > that even low levels of aerobic exercise, can be very stressful, and

> > sometimes even unendurable.

 

It would seem to me he could get some benefit from using some oxygen

with exercise. I have not gone overboard using oxygen. Typically, I only

do this once per week. Within the last six months, I have only used oxygen

twice within one week.

 

I am talking 10 to 15 minutes on the oxygen. Since there is no way

to escape the free radical dilemma, and with oxygen it is worse, so.... we

need to use more of the supplements before exercise.

 

I do seriously believe the oxygen helped me. When I first started

this, I could only walk on the treadmill for 6 to 8 minutes. Within about

3 or 4 weeks, I could continue for 25, 35, and even 45 minutes.

 

Interesting.... this was in the springtime when I was eating tons of

fresh raw stuff, cottage cheese, flax oil, and following the Dr. Pauling

program.

 

Maybe it was all this other stuff, and I was dead wrong thinking the

oxygen helped.

 

I can't wait until spring to try it all over again. Of course not

getting the sunlight in winter could make one weaker and not be able to

feel like he could walk to the top of the mountain.

 

Still a bit confused on the use of oxygen..........

 

Wayne

[Guest guest]

-

" Wayne Fugitt " <wayne

 

Monday, January 21, 2002 10:52 AM

Re: Ascorbic acid inhibits the increase in low-density

lipoprotein (LDL) susceptibility to

oxidation and the proportion of electronegative LDL induced by intense aerobic

exercise.

 

 

> Still a bit confused on the use of oxygen..........

 

Hi Wayne,

 

Sure using supplemental oxygen will boost the level of exercise you can do at

your current fitness level and make you

think you are at a higher fitness level than you really are. But to what

reason? You may overstress your bodies

systems.

 

To limit Cortisol buildup, exercise should not be done more than 30 minutes.

Aerobic is fine for boosting the bodies

ability to supply oxygen rich blood to the cells and carbon dioxide rich blood

to the lungs but only weight bearing

exercise will build muscles and if you can do 10 reps the weight is too light.

 

1 g Vit C and 500 IUs Vit E before exercise and 2 g Glutamine after to speed

recovery works well for me.

========================

Good Health & Long Life,

Greg Watson, http://optimalhealth.cia.com.au gowatson

USDA database (food breakdown) http://www.nal.usda.gov/fnic/foodcomp/

PubMed (research papers) http://www.ncbi.nlm.nih.gov/entrez/query.fcgi

DWIDP (nutrient analysis) http://www.walford.com/dwdemo/dw2b63demo.exe

Patch file for above http://www.walford.com/download/dwidp67u.exe

KIM (omega analysis) http://ods.od.nih.gov/eicosanoids/KIM_Install.exe

[Guest guest]

Wayne

I was intrigued by what you said concerning combining the use of oxygen, the

Budwig protocol, and the Pauling system. What struck me was the sharp

increase in your treadmill time. I usually am lucky, due to angina, to get

to 6 minutes on a treadmill, even at very slow speed. I'm currently taking

a Pauling prep. containing Lysine, Proline, C,etc. to see the outcome.

The problem of mixing therapeutic modalities, is easy to fall into. You

aren't sure it was the oxygen therapy that did the trick, in allowing you to

go between 25 to 45 min's on the treadmill. Perhaps you could restrain

yourself from doing it all at once, in the springtime, and try these various

therapies, consecutively. Of course, there's the problem of not knowing how

long one has to stay on any of these systems to have given each, a

reasonable chance to do its work. By the way, do you experience angina

during prolonged aeorobics, or is it other symptoms? The definition of

angina is a bit murky. It means, literally, heart-pain, and, by extension,

usually left arm, jaw, etc. pain; but is also used, I think mistakenly, to

include " pressure " or " tightness " in the chest, dizziness, or shortness of

breath, which, I feel should be separately defined symptoms of ischemia

(deprivation of oxygen to the heart muscle).

In any case, I hope you keep us posted about your progress along these

lines.

-

" Wayne Fugitt " <wayne

 

Sunday, January 20, 2002 4:22 PM

Re: Ascorbic acid inhibits the increase in

low-density lipoprotein (LDL) susceptibility to oxidation and the proportion

of electronegative LDL induced by intense aerobic exercise.

 

 

> Evening Greg,

>

> >Exercise increases free radical production. The more intense the

> >exercise, the more free radicals are generated.

>

> So there is no way to avoid this? The best thing to do is take the

> addition supplements before the exercise session, right.

>

> Still, we have to face a bit of this " free radical generation " , or else

> we do no exercise. This must mean there is some

> magic amount of exercise that is right for us.

>

> Your logic on this issue does fill in the gaps on many things I have

> read written by doctors. They however, did not explain the full details

> and what should be done to lessen the effect of the free radicals.

>

> You also pointed out that when I exercise with oxygen, I make this

> worse. Surely, if you try real hard, you can think of some benefit to

> doing exercise with oxygen.

>

>

> In Johns case.......

>

> > > to be much lower levels of exercise intensity. I suffer from angina,

so

> > > that even low levels of aerobic exercise, can be very stressful, and

> > > sometimes even unendurable.

>

> It would seem to me he could get some benefit from using some oxygen

> with exercise. I have not gone overboard using oxygen. Typically, I

only

> do this once per week. Within the last six months, I have only used

oxygen

> twice within one week.

>

> I am talking 10 to 15 minutes on the oxygen. Since there is no way

> to escape the free radical dilemma, and with oxygen it is worse, so.... we

> need to use more of the supplements before exercise.

>

> I do seriously believe the oxygen helped me. When I first started

> this, I could only walk on the treadmill for 6 to 8 minutes. Within about

> 3 or 4 weeks, I could continue for 25, 35, and even 45 minutes.

>

> Interesting.... this was in the springtime when I was eating tons of

> fresh raw stuff, cottage cheese, flax oil, and following the Dr. Pauling

> program.

>

> Maybe it was all this other stuff, and I was dead wrong thinking the

> oxygen helped.

>

> I can't wait until spring to try it all over again. Of course not

> getting the sunlight in winter could make one weaker and not be able to

> feel like he could walk to the top of the mountain.

>

> Still a bit confused on the use of oxygen..........

>

> Wayne

>

>

>

>

>

> Getting well is done one step at a time, day by day, building health

> and well being.

>

> To learn more about the Gettingwell group,

> Subscription and list archives are at:

> Gettingwell

>

>

[Guest guest]

Greg

Concerning the buildup of " cortisol, " and your limit of 30 minutes for

exercise; does that limit apply to both aerobic and an-aerobic exercise,

either of them alone, or in combination?

John P.

-

" Greg Watson " <gowatson

 

Sunday, January 20, 2002 3:15 PM

Re: Ascorbic acid inhibits the increase in

low-density lipoprotein (LDL) susceptibility to oxidation and the proportion

of electronegative LDL induced by intense aerobic exercise.

 

 

> -

> " John Polifronio " <counterpnt

>

> Sunday, January 20, 2002 12:25 AM

> Re: Ascorbic acid inhibits the increase in

low-density lipoprotein (LDL) susceptibility to

> oxidation and the proportion of electronegative LDL induced by intense

aerobic exercise.

>

>

> > Greg

> > Thank you for these fascinating findings. Notice that the paper states

that

> > this increase in oxidized LDL is seen during " intense " aerobic exercise.

> > But this leaves unclarified, the matter of " level of fitness, " in

> > determining what constitutes " intense " exercise. I'm guessing that

people

> > in ill health, at low levels of fitness, will probably experience this

> > increase in LDL oxidation at what appear, to the robustly healthy

indivdual,

> > to be much lower levels of exercise intensity. I suffer from angina, so

> > that even low levels of aerobic exercise, can be very stressful, and

> > sometimes even unendurable.

>

> Hi John,

>

> Exercise increases free radical production. The more intense the

exercise, the more free radicals are generated. I now

> take both 1 g Vit C and 500 IUs Vit E before by exercises with 1

tablespoon of VCNO and 2 g Glutamine after to speed

> recovery. I never exercise longer than 30 minutes as the catabolic and

pro-aging hormone Cortisol slowly builds up.

> ========================

> Good Health & Long Life,

> Greg Watson, http://optimalhealth.cia.com.au gowatson

> USDA database (food breakdown) http://www.nal.usda.gov/fnic/foodcomp/

> PubMed (research papers) http://www.ncbi.nlm.nih.gov/entrez/query.fcgi

> DWIDP (nutrient analysis) http://www.walford.com/dwdemo/dw2b63demo.exe

> Patch file for above http://www.walford.com/download/dwidp67u.exe

> KIM (omega analysis)

http://ods.od.nih.gov/eicosanoids/KIM_Install.exe

>

>

>

>

> Getting well is done one step at a time, day by day, building health

> and well being.

>

> To learn more about the Gettingwell group,

> Subscription and list archives are at:

> Gettingwell

>

>

[Guest guest]

-

" John Polifronio " <counterpnt

 

Monday, January 21, 2002 12:46 PM

Re: Ascorbic acid inhibits the increase in low-density

lipoprotein (LDL) susceptibility to

oxidation and the proportion of electronegative LDL induced by intense aerobic

exercise.

 

 

> Greg

> Concerning the buildup of " cortisol, " and your limit of 30 minutes for

> exercise; does that limit apply to both aerobic and an-aerobic exercise,

> either of them alone, or in combination?

 

Hi John,

 

Either, in any combo. Once cortisol build up, it strips your muscles apart to

get at protein, to turn into amino acids,

to feed the liver, to make more glycogen.

 

You get rapidly declining benefits going beyond 30 minutes.

========================

Good Health & Long Life,

Greg Watson, http://optimalhealth.cia.com.au gowatson

USDA database (food breakdown) http://www.nal.usda.gov/fnic/foodcomp/

PubMed (research papers) http://www.ncbi.nlm.nih.gov/entrez/query.fcgi

DWIDP (nutrient analysis) http://www.walford.com/dwdemo/dw2b63demo.exe

Patch file for above http://www.walford.com/download/dwidp67u.exe

KIM (omega analysis) http://ods.od.nih.gov/eicosanoids/KIM_Install.exe