Caloric restriction of rhesus monkeys lowers oxidative damage in skeletal muscle.

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Caloric restriction of rhesus monkeys lowers oxidative damage in skeletal

muscle.

Zainal TA, Oberley TD, Allison DB, Szweda LI, Weindruch R.

Departments of Nutritional Sciences, Pathology and Laboratory Medicine,

Medicine, Wisconsin Regional Primate Research

Center, University of Wisconsin, Madison, Wisconsin 53705, USA.

 

In laboratory rodents, caloric restriction (CR) retards several age-dependent

physiological and biochemical changes in

skeletal muscle, including increased steady-state levels of oxidative damage to

lipids, DNA, and proteins.

 

We used immunogold electron microscopic (EM) techniques with antibodies raised

against 4-hydroxy-2-nonenal

(HNE) -modified proteins, dinitrophenol, and nitrotyrosine to quantify and

localize the age-dependent accrual of

oxidative damage in rhesus monkey vastus lateralis skeletal muscle.

 

Using immunogold EM analysis of muscle from rhesus monkeys ranging in age from 2

to 34 years old, a fourfold maximal

increase in levels of HNE-modified proteins was observed.

 

Likewise, carbonyl levels increased approximately twofold with aging.

 

Comparing 17- to 23-year-old normally fed to age-matched monkeys subjected to CR

for 10 years, levels of HNE-modified

proteins, carbonyls, and nitrotyrosine in skeletal muscle from the CR group were

significantly less than control group

values.

 

Oxidative damage largely localized to myofibrils, with lesser labeling in other

subcellular compartments.

 

Accumulation of lipid peroxidation-derived aldehydes, such as malondialdehyde

and 4-hydroxy-2-alkenals, and protein

carbonyls were measured biochemically and confirmed the morphological data.

 

Our study is the first to quantify morphologically and localize the

age-dependent accrual of oxidative damage in

mammalian skeletal muscle and to demonstrate that oxidative damage in primates

is lowered by CR.

 

PMID: 10973932 [PubMed - indexed for MEDLINE]

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