Omega 3 - Big benefits

[Guest guest]

Hi All

 

Here is some interesting text from Pub Med on DHA

Note its dose dependent action!

Fish oil strong in DHA is worth looking at, maybe even learning to

like cod liver oil. :-) :-)

 

Rick

 

 

Biochim Biophys Acta 2001 Jan 15;1499(3):265-75

Docosahexaenoic acid induces apoptosis in Jurkat cells by a

protein phosphatase-mediated process.

 

Siddiqui RA, Jenski LJ, Neff K, Harvey K, Kovacs RJ, Stillwell W.

 

Cellular Biochemistry Laboratory, Methodist Research Institute at Clarian

Health,

Indianapolis, IN 46202, USA. rsiddiqu

 

Docosahexaenoic acid (DHA) is an omega-3 fatty acid under intense investigation

for its ability to modulate cancer cell growth and survival. This research was

performed to study the cellular and molecular effects of DHA. Our experiments

indicated that the treatment of Jurkat cells with DHA inhibited their survival,

whereas

similar concentrations (60 and 90 microM) of arachidonic acid and oleic acid had

little effect. To explore the mechanism of inhibition, we used several measures

of

 

apoptosis to determine whether this process was involved in DHA-induced cell

death

 

in Jurkat cells. Caspase-3, an important cytosolic downstream regulator of

apoptosis,

is activated by death signals through proteolytic cleavage. Incubation of Jurkat

cells

with 60 and 90 microM DHA caused proteolysis of caspase-3 within 48 and 24 h,

respectively. DHA treatment also caused the degradation of poly-ADP-ribose

polymerase and DNA fragmentation as assayed by flow cytometric TUNEL (terminal

deoxynucleotidyl transferase-mediated dUTP nick end labeling) assay. These

results

 

indicate that DHA induces apoptosis in Jurkat leukemic cells. DHA-induced

apoptosis was effectively inhibited by tautomycin and cypermethrin at

concentrations

that affect protein phosphatase 1 (PP1) and protein phosphatase 2B (PP2B)

activities, respectively, implying a role for these phosphatases in the

apoptotic

pathway. Okadaic acid, an inhibitor of protein phosphatase 2A, had no effect on

DHA-induced apoptosis. These results suggest that one mechanism through which

DHA may control cancer cell growth is through apoptosis involving PP1/PP2B

protein

 

phosphatase activities.

 

PMID: 11341974 [PubMed - indexed for MEDLINE]

 

 

 

 

[Guest guest]

--- Rick Muenzer <r.muenzer wrote:

> Here is some interesting text from Pub Med on

> DHA

> Note its dose dependent action!

> Fish oil strong in DHA is worth looking at, maybe

> even learning to

> like cod liver oil. :-) :-)

>

And it has 500 mg per mere teaspoon! :-) :-)

 

Pam

 

 

 

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